Adipoqcre mt/mg idtr mice

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WebAim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ERT2), which revealed age, sex and genotype as factors impacting on non-induced Cre recombination. ABSTRACT Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to … WebSep 20, 2011 · Selective ablation of lysozyme M-positive (LysM(+)) myelomonocytic cells by low-dose diphtheria toxin in mice with inducible expression of the diphtheria toxin receptor (LysM(iDTR) mice) reduced the number of monocytes in the circulation and limited ATII-induced infiltration of these cells into the vascular wall, whereas the number of ...

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WebMay 17, 2024 · Lineage negative (CD45- & CD31-) cells were isolated from uninjured skin and day 5 dorsal skin wound beds of 7-week-old AdipoqCre; mT/mG mice using FACS. This experiment describes multiple subsets of wound bed myofibroblasts and identifies that Adipoq-traced cells contribute to the myofibroblast pool in wound beds. Overall design WebApr 16, 2024 · To determine if the Adipoq promoter is active in other cell types prior to injury, we analyzed GFP+ expression in AdipoqCre+; mT/mG mice and AdipoqCreER; mT/mG … pickles bottle

007576 - mT/mG , mTmG Strain Details - The Jackson Laboratory

WebSep 4, 2024 · While mGFP expression was minimal in adipocytes of untreated Adipoq-CreER; mT/mG mice (0.17% of perilipin+ cells expressed mGFP) (Supplementary Fig. 4a) or in APs from Adipoq-Cre; mT/mG... WebJan 4, 2024 · Using either Cx3cr1-iDTR mice in combination with Dtx or via the PLX5622 diet to pharmacologically ablate microglia, we compared the two models via MRI and histology to study the general anatomy of the brain and the CSF/ventricular systems. ... mice were treated with vehicle or Repertaxin at 6 h after each Dtx injection at a dose of … WebNational Center for Biotechnology Information pickles broodjes asse

Mouse Models of Lipodystrophy and Their Significance …

Unspecific DNA recombination in AdipoqCre-ERT2

WebOct 1, 2010 · Adipoq encodes adiponectin, an adipokine specifically expressed in differentiated adipocytes. Tamoxifen treatment induced almost complete recombination in white adipose tissue of the AdipoqCreER... WebAdipoq-Cre BAC transgenic mice are a Cre-lox tool for deletion of floxed sequences in white adipose tissue and brown adipose tissue, but not in activated or resident macrophages. … pickles bookWebMay 20, 2005 · Our results validate the use of iDTR mice as a tool for cell ablation in vivo. ... (0.4 mg/ml; Roche) and optical density (OD) measurements at 405 nm versus 570 nm were compared to standard curves ... top 50 companies in thailand 2022

"WebCreERT2xTDTO mice, we observed the previously described Cre leakage without TAM administration. Therefore, we aimed at analysing non-induced DNA recombination in Adipoq-CreERT2xTDTO mice in a more detailed manner using mice of different genotype, sex and age. Importantly, we found that leakage of Cre-ERT2 recombination in Adipoq … " - Adipoqcre mt/mg idtr mice

Adipoqcre mt/mg idtr mice

(PDF) MTOR-mediated podocyte hypertrophy regulates

WebApr 30, 2024 · To date, a dozen mouse models for mast cell deficiency have been reported. However, mice with a specific depletion of all populations of mast cells have not been reported. We generated knock-in mice, termed Mcpt5 / Cma1DTR mice, expressing human diphtheria toxin A (DT) receptor under the endogenous promoter of Mcpt5 (also known … WebDec 17, 2015 · Human diphtheria toxin receptors (DTR) were selectively expressed in parathyroid cells by using mice that carry an iDTR allele. 24 These mice were mated to mice that express the Cre allele under the control of the human 5.5-kb PTH promoter, which is active only in parathyroid cells. 23 In the resulting PTHcre-iDTR mice, parathyroid …

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WebThe following mice (catalog numbers are from Jackson Laboratory, Bar Harbor, ME, USA) were obtained: ROSAmT/mG mice (007676),(22) PTH-Cre mice (005989),(23) and Cre-inducible DTR transgenic mice (iDTR) mice (007900).(24) Genotyping was performed on genomic DNA isolated from tails, using previously publishedprotocols ... WebJan 25, 2024 · Male 2-mo-old Pdgfrα mT/mG or Pdgfrα iDTR;mT/mG mice were sequentially administered TM and DTX (TM: 150 mg·kg-1 per day for 5 d; DTX: 45.7 μg·kg-1 per day for 3 d starting 10 d after TM).

WebDue to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER T2).We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter … WebMay 18, 2015 · Wanner et al. crossed the iDTR strain to hNPHS2.rtTA, TetO.Cre, and mT/mG mice to specifically target podocyte ablation, in a dose-dependent manner, and determine the influence of acute podocyte loss on podocyte regeneration (Figure 3(b)).

WebWe used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ERT2), which were crossed to a tdTomato reporter mouse to … WebSep 26, 2024 · Next, we assessed the effects of deleting Mks to understand their impact in acute thrombocytopenia using Pf4 (CXCL4)-cre; iDTR mice. 6,7 Three days after DT induction, Mks were depleted in Pf4-cre +; iDTR mice, but the number of platelets in the circulation was not reduced in Mk-deficient mice compared with DT-treated Cre − …

WebSep 19, 2024 · By 9 weeks, mice with podocyte-specific deletion of Vps34 had developed early proteinuria, progressive glomerulosclerosis, and renal failure. ... Autophagy as a Therapeutic Target for Chronic...

Web007576 STOCK Gt(ROSA)26Sor tm4(ACTB-tdTomato,-EGFP)Luo /J mT/mG (also called ROSA mT/mG ) is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages … top 50 companies in india nseWebApr 1, 2024 · Given their anatomical location, it is unsurprising that dermal adipocytes also play a key part in wound healing. Using a murine model with an inducible diphtheria toxin … top 50 companies in kolkataWebJun 20, 2014 · Adipocyte dysfunction is believed to underlie the development of metabolic disease in obesity. Hence, understanding how one might beneficially manipulate adipose … pickles bricklayingWebHere we describe mT/mG, a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer Tomato (mT) prior to Cre-mediated excision and … top 50 companiesWebNational Center for Biotechnology Information top 50 companies in sri lanka 2017WebAdipoqCre+;mT/mG and AdipoqCreER+;mT/mG mice given tamoxifen 7 days before analysis. (B) Flow cytometry plot of Pdgfra colocalization in cell populations from … pickles bread and butter recipeWebSep 4, 2024 · While mGFP expression was minimal in adipocytes of untreated Adipoq-CreER; mT/mG mice (0.17% of perilipin+ cells expressed mGFP) (Supplementary Fig. … We would like to show you a description here but the site won’t allow us. pickles brands