WebApr 11, 2024 · The PCR program consisted of 3 min at 95°C, followed by 35 cycles of 15 s at 95°C, 15 s at 55°C, and 120 s at 72°C, followed by a 5 min extension at 72°C. The PCR product was inserted into the pEASY-Blunt Simple Cloning vector (TransGen Biotech, Beijing, China) and then transformed into DH5α for sequencing. Web茶树磷酸烯醇式丙酮酸转运子csppt2基因的克隆和分析. 2024-04-22 纪志芳 甘玉迪 陈常颂 杨鼎俊 孙康 黎星辉 陈暄
Cloning, characterization and prokaryotic expression analysis of …
WebMay 10, 2024 · Cloning and expression of the C11orf96 protein The C11orf96 gene in the bait vector pEASY®-Blunt Zero Cloning Kit (abbreviated as pEBCK) was obtained by RT-PCR, and 1% agarose gel electrophoresis showed that the size of the cDNA fragment amplified by PCR was in the expected range. WebpEASY®-T5 Zero T/A TOPO Cloning Kit. pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment containing 3’A-overhangs) disrupts the expression of the gene. ... pEASY-Blunt Zero Cloning Kit – CB501 $ 338.00 – $ 898.00 CAD; EasyPure® HiPure (Endo-free) Plasmid MiniPrep Kit – EM111 $ 105.00 CAD; Trans1-Blue ( XL1 ... ikea monthly payment
拟南芥抗旱相关基因的表达载体构建及转基因植株鉴定_参考网
WebDec 6, 2024 · About 1-cm-long root segments were incubated in 10% KOH solution at 95°C for 100 min, rinsed with distilled water, bleached with 10% hydrogen peroxide solution for 15 min, acidified with 0.2 mol/L hydrochloric acid for 10 min, and stained with 0.05% trypan blue in lactate phenol for 1 min. WebDetails. 6027. Mighty Cloning Reagent Set (Blunt End) 20 Rxns. USD $377.00. Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products … WebHigh cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for short and large fragment cloning. Kanamycin and Ampicillin resistance genes … ikea moon picture